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huHSC-NKG-ProM Mouse
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huHSC-NKG-ProM Mouse
제품명
huHSC-NKG-ProM Mouse
제품 ID
C001544
품종 계통
huHSC-ProM-NOD.Cg-PrkdcscidIl2rgem1cya/Cya
Backgroud
NKG
상태
이 마우스 계통을 논문에서 사용할 경우, “huHSC-NKG-ProM Mouse (카탈로그 번호 C001544)은 Cyagen에서 구입하였습니다.”라고 명시해 주시기 바랍니다.
Immunodeficient Mice
Humanized Immune System Models
구매 가능한 제품 종류
연령
Genotype
성별
수량
표준 제공 조건은 최소 3마리의 이형접합(heterozygous) 보균자를 보장합니다. 동형접합(homozygous) 보균자 및/또는 특정 성별에 대한 브리딩 서비스도 제공됩니다.
가격 문의
Immunodeficient Mice
Humanized Immune System Models
기본 정보
검증 데이터
관련 자료
기본 정보
유전자명
유전자 별칭
gc, p64, [g]c, CD132, gamma(c)
NCBI ID
염색체
Chr X
MGI ID
Datasheet
품종 계통 설명
NKG mice (Catalog number: C001316) are severely immunodeficient mice generated by Cyagen through deleting the Il2rg gene from NOD-Scid mice. NKG mice exhibit deficiency of mature T cells, B cells, and functional NK cells, reduced complement activity, and weak phagocytosis of human-derived cells by macrophages, which are well suited for the transplantation of human hematopoietic stem cells (HSC), peripheral blood mononuclear cells (PBMC), adult stem cells and tissues, and patient-derived xenografts (PDX).
huHSC-NKG mice are immune system humanized mouse models constructed by transplanting human hematopoietic stem cells (HSC) into NKG mice after sub-lethal dose irradiation. This model has a long lifespan and can successfully reconstitute various immune cells. In addition, huHSC-NKG mice typically do not develop graft-versus-host disease (GvHD) and the reconstitution period can be long.
The huHSC-NKG-ProM mouse is the latest enhanced version of the huHSC-NKG series, where "Pro" indicates the enhanced series and "M" specifically refers to myeloid cells. This mouse utilizes advanced neonate technology and has been process-optimized to allow for the development of lymphoid T cells and B cells, as well as myeloid cells such as monocytes. It is suitable for research on immune checkpoint inhibitors and drugs that target myeloid cells.
응용 분야
Tumor Immunology Research: Suitable for drug types such as immune checkpoint inhibitors and those with targets on myeloid cells, for example, combining a PD-1 monoclonal antibody with drugs that affect Macrophage polarization;
Autoimmune Disease Research: Evaluation of the pathogenesis of autoimmune diseases and potential therapeutic strategies;
Drug Metabolism and Toxicity Research: Assessment of the pharmacokinetic properties and toxic effects of candidate compounds in vivo.
검증 데이터
1. Survival and Growth Curves
During the process of human immune system reconstitution, the body weight of NKG mice was maintained at 18-22 grams, and the survival rate at 18 weeks post-reconstitution was 85%.
Figure 1. Survival and body weight changes in NKG mice following human hematopoietic stem cells (HSCs) transplantation.
2. Reconstitution of Human Leukocytes in Peripheral Blood
Transplantation of human umbilical cord blood-derived hematopoietic stem cells (HSCs) into NKG mice for human immune system reconstitution resulted in a stable reconstitution efficacy, with the proportion of human-derived CD45+ leukocytes reaching approximately 40%-60%.
Figure 2. Reconstitution of human CD45+ leukocytes in the peripheral blood of NKG mice following transplantation of human HSCs.
3. Reconstitution of Human T Cells and B Cells in Peripheral Blood
The gradual reconstruction of T cells commenced from the 10th week post-transplantation of human hematopoietic stem cells (HSCs), with the mean T cell content reaching 40% by the 16th week. The proportion of B cells ranged from approximately 60-80% during weeks 6-8 post-reconstruction. Subsequently, as the T cell proportion increased, the B cell proportion gradually decreased to 40-60%.
Figure 3. Reconstitution of human T cells and B cells in the peripheral blood of NKG mice following transplantation of human HSCs.
4. Reconstitution of Human NK Cells and Monocytes in Peripheral Blood
The average reconstitution proportion of Natural Killer (NK) cells was below 5%, while monocytes in peripheral blood achieved a reconstitution proportion of 5-10%. Given that macrophages are primarily derived from monocytes, monocyte detection is therefore the main focus in peripheral blood samples.
Figure 4. Reconstitution of human NK cells and monocytes in the peripheral blood of NKG mice following transplantation of human HSCs.
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